This time-lapse movie describes the steps that need to be undertaken to set up the assay as previously described (Palanivelu & Preuss (2006), BMC Plant Biology, 6:7). The assay can be used to monitor pollen tube-ovule interactions. Since the assay is modular, any combination of the three components of the assay (pollen, stigma/style and ovules) can be tested, including genetically impossible combinations such as the behavior of wild type pollen tubes grown through a wild-type pistil with mutant ovules.