Time-lapse imaging of DsRED-tagged pollen tubes, entering an ovule and reaching the portion of the ovule where synergid is expected to be located. The pollen tube remains without discharge within the ovule, as evident from the lack of a visible fluorescent spot. This is a representative movie of instances in a gfa2/GFA2 ovule where the pollen tube did not discharge. Time elapsed between each frame = 10 minutes.
Reference: Leydon, A. R., Tsukamoto, T., Dunatunga, D., Qin, Y., Johnson, M. A., and Palanivelu, R. (2015). Pollen tube discharge completes the process of synergid degeneration that is initiated by pollen tube – synergid interaction in Arabidopsis. Plant Physiology 169: 485-496. Link to the article.