Growing Arabidopsis pollen tubes by the semi in vivo (SIV) method Pollen Growth Protocol – Download PDF Illustration of this protocol:.avior .mov Ref: Palanivelu & Preuss (2006). BMC Plant Biology, 6:
Growing Arabidopsis pollen tubes in vitro In-vitro pollen growth protocol – Download PDF Additional papers on growing Arabiodopsis pollen tubes in vitro: A. Boavida and McCormick (2007), Plant Journal, 52: 570–582 – Download PDF B. Firas Bou Daher, Youssef Chebli and Anja Geitmann (2009), Plant Cell Reports 28 (3): 347-357 – Download PDF C. M. J. Rodriguez-Enriquez, S. Mehdi, H. G. Dickinson, and R. T. Grant-Downton (2013) New Phytologist 197: 668–679 doi: 10.1111/nph.12037 – Download PDF. Cellophane used in this method not only allows near 100% germination but also allows ready peeling off pollen tubes after growth and placing them on a slide for easy imaging.
Protocol to prepare pollen tubes for observation by optical and electron microscopy Chebli et al (2008), Microscopical Society of Canada bulletin 36(3) : 15-19 – Download PDF
Protocol for Auramine O staining of pollen exine Auramine O staining of pollen exine – Download PDF Ref: Dobritsa et al (2009) Plant Physiology 151(2): 574–589
Protocol for confocal imaging of ovules, female gametophyte, and fertilized seeds Confocal microscope-based autofluorescence assay – Download PDF Ref: Christensen et al (1997) Sex. Plant Reprod. 10, 49-64