Video 2. Pollen tube migration within an ovule in an in vitro guidance assay

Time-lapse images showing a GFP-tagged tube entering and navigating within the ovule. The growth rate of the pollen tube decreases substantially before and soon after entry. Within the ovule, pollen tube ceases growth presumably in one of the synergids and tube discharge occurs, leaving a visible GFP spot. Ref: Palanivelu & Preuss (2006), BMC Plant Biology;…Continue Reading Video 2. Pollen tube migration within an ovule in an in vitro guidance assay

Video 1. In vitro pollen tube guidance assay monitors ovule targeting by pollen tubes.

Time-lapse imaging of GFP-tagged pollen tubes emerging from the cut portion of the pistil, traveling across the agarose medium before approaching a subset of excised ovules. In this instance, pollen tubes grew within 100 µm only near two of the six ovules; of these two, only one was successfully targeted. Ref: Palanivelu & Preuss (2006), BMC…Continue Reading Video 1. In vitro pollen tube guidance assay monitors ovule targeting by pollen tubes.