Videos

Time-lapse imaging of GFP-tagged pollen tubes emerging from the cut portion of the pistil, traveling across the agarose medium before approaching a subset of excised ovules. In this instance, pollen tubes grew within 100 µm only near two of the six ovules; of these two,…Continue Reading Video 1. In vitro pollen tube guidance assay monitors ovule targeting by pollen tubes.

Time-lapse images showing a GFP-tagged tube entering and navigating within the ovule. The growth rate of the pollen tube decreases substantially before and soon after entry. Within the ovule, pollen tube ceases growth presumably in one of the synergids and tube discharge occurs, leaving a…Continue Reading Video 2. Pollen tube migration within an ovule in an in vitro guidance assay

Three pollen tubes approach an ovule, however only one of them is ultimately successful. The unsuccessful tubes stall near the micropyle, despite coming very close to it. Ref: Palanivelu & Preuss (2006), BMC Plant Biology 6:7….Continue Reading Video 3. Pollen tube repulsion by a targeted ovule in an in vitro guidance assay

Three pollen tubes approach an ovule, however, the pollen tube that arrived at the micropyle first was successful. The unsuccessful tubes approached the micropyle; however, one of them turned away from it, while the other one skips the micropyle and grows over the successful tube…Continue Reading Video 4. Pollen tube repulsion by a targeted ovule in an in vitro guidance assay

Another example of a late-arriving pollen tube not being able to enter an ovule that is already targeted. Ref: Palanivelu, R. (Unpublished)…Continue Reading Video 5. Pollen tube repulsion in an in vitro guidance assay

Three pollen tubes approach an ovule, however only one of them is ultimately successful. The two unsuccessful tubes turn dramatically away from the micropyle and move away from the ovule. Ref: Palanivelu, R. (Unpublished)…Continue Reading Video 6 . Pollen tube repulsion by a targeted ovule in an in vitro guidance assay

Time-lapse imaging of DsRed-tagged pollen tubes entering the ovule and traveling within the ovule before approaching the GFP-tagged synergid cell within the female gametophyte. Left video, time-lapse images captured in the red and green channels. Right video, time-lapse images captured in the green channel only….Continue Reading Video 7. Pollen tube interaction with the synergid cell in an in vitro guidance assay

Time-lapse imaging of DsRed-tagged pollen tubes entering the ovule and traveling within the ovule before approaching the GFP-tagged synergid cell within the female gametophyte. Left video, time-lapse images captured in the red and green channels. Right video, time-lapse images captured in the green channel only….Continue Reading Video 8. Pollen tube interaction with the synergid cell in an in vitro guidance assay

Time-lapse imaging of DsRed-tagged pollen tubes entering the ovule and traveling within the ovule before approaching the GFP-tagged synergid cell within the female gametophyte. Left video, time-lapse images captured in the red and green channels. Right video, time-lapse images captured in the green channel only….Continue Reading Video 9. Pollen tube interaction with the synergid cell in an in vitro guidance assay

Time-lapse imaging of DsRed-tagged pollen tubes entering the ovule and traveling within the ovule before approaching the GFP-tagged synergid cell within the female gametophyte. Left video, time-lapse images captured in the red and green channels. Right video, time-lapse images captured in the green channel only….Continue Reading Video 10. Pollen tube interaction with the synergid cell in an in vitro guidance essay

Time-lapse imaging of LAT52:DsRed-tagged pollen tube entering a wild-type ovule and traveling within the ovule before arresting growth and bursting in the DD3:GFP-marked synergid cell. The targeted ovule repels the second, late-arriving pollen tube away from the ovule micropyle. The video shows merged time-lapse images captured in…Continue Reading Video 11. Pollen tube interaction with a wild-type ovule in an in vitro guidance assay

Time-lapse imaging of LAT52:DsRed-tagged pollen tube entering a lre-5/lre-5 ovule, reaching DD3:GFP-marked synergid cell, and undergoing normal pollen tube reception. The video shows merged time-lapse images captured in red and green channels. Time elapsed between two frames is 10 minutes. Ref: Tsukamoto, T., Qin Y., Huang, Y., Dunatunga, D. and…Continue Reading Video 12. Pollen tube interaction with a lre-5/lre-5 ovule that induces normal pollen tube reception in an in vitro guidance assay

Time-lapse imaging of a LAT52:DsRed-tagged pollen tube entering a lre-5/lre-5 ovule, reaching a DD3:GFP-marked synergid cell, continuing to grow incessantly in the synergid cell, and eventually growing further into the female gametophyte. The movie shows merged time-lapse images captured in red and green channels. The time elapsed between two…Continue Reading Video 13. Pollen tube reception defect in a lre-5/lre-5 ovule is recapitulated in an in vitro guidance assay

Time-lapse imaging of a LAT52:DsRed-tagged pollen tube entering a lre-5/lre-5 ovule, reaching a DD3:GFP-marked synergid cell, continuing to grow incessantly in the synergid cell, and eventually growing further into the female gametophyte. The movie shows merged time-lapse images captured in red and green channels. The time elapsed between two…Continue Reading Video 14. Multiple pollen tubes enter a single are-5/lre-5 ovule in an in vitro guidance assay

Time-lapse imaging of Arabidopsis PTs emerging from pollen grains deposited directly on the pollen growth medium. Time elapsed between two frames is indicated in minutes (min) on the top left hand corner. Reference: Palanivelu, R., and Johnson, M.A. (2010). Functional genomics of pollen tube-pistil interactions in…Continue Reading Video 15. In vitro pollen tube growth

Time-lapse imaging of Arabidopsis PTs emerging from pollen grains deposited directly on the pollen growth medium. Time elapsed between two frames is indicated in minutes (min) on the top left hand corner. Reference: Palanivelu, R., and Johnson, M.A. (2010). Functional genomics of pollen tube-pistil interactions in…Continue Reading Video 16. Semi-in vivo (SIV) procedure for growing pollen tubes

Time-lapse imaging of Arabidopsis pollen tubes emerging from the cut portion of a pistil, fanning out, and traveling across the pollen growth medium filled in a microchannel, and then the tubes respond to ovules placed in a microchamber. The cartoon below shows the setup for…Continue Reading Video 17. Microfluidic procedure for growing Arabidopsis pollen tubes.

Time-lapse imaging of Arabidopsis pollen (Columbia accession) germination on a solid pollen growth medium. Pollen grains from the same anther were spotted proximal to three holes: one filled with 50 μM N-Methanesulfinyl 1-azadecalin, a second one filled with 50 μM N-Methanesulfinyl 2-azadecalin, and a third…Continue Reading Video 18. Stimulation of pollen (Columbia accession) germination by sulfinylated azadecalins.

Time-lapse imaging of Arabidopsis pollen (Landsberg accession) germination on a solid pollen growth medium. Pollen grains from the same anther were spotted proximal to three holes, one filled with 50 μM N-Methanesulfinyl 1-azadecalin, a second one filled with 50 μM N-Methanesulfinyl 2-azadecalin and a third…Continue Reading Video 19. Stimulation of pollen (Landsberg accession) germination by sulfinylated azadecalins.

This time-lapse movie describes the steps that need to be undertaken to set up the assay as previously described (Palanivelu & Preuss (2006), BMC Plant Biology, 6:7). The assay can be used to monitor pollen tube-ovule interactions. Since the assay is modular, any combination of…Continue Reading Video 20. Description of the Arabidopsis semi-in vivo pollen tube guidance assay.

Time-lapse imaging of DsRED-tagged pollen tubes, entering an ovule and reaching the portion of the ovule where synergid is expected to be located. Within the ovule, pollen tube discharge occurs presumably in one of the synergids, leaving a visible fluorescent spot. Time elapsed between each…Continue Reading Video 21. A semi in vivo pollen tube guidance assay to monitor pollen tube discharge in wild-type ovules.

Time-lapse imaging of DsRED-tagged pollen tubes entering an ovule and reaching the portion of the ovule where a synergid is expected to be located. Within the ovule, apparently, normal pollen tube discharge occurs, presumably in one of the synergids, leaving a visible fluorescent spot. This is…Continue Reading Video 22. A semi in vivo pollen tube guidance assay to monitor pollen tube discharge in gfa2/GFA2 ovules.

Time-lapse imaging of DsRED-tagged pollen tubes, entering an ovule and reaching the portion of the ovule where synergid is expected to be located. The pollen tube remains without discharge within the ovule, as evident from the lack of a visible fluorescent spot. This is a representative movie…Continue Reading Video 23. A semi-in vivo pollen tube guidance assay to monitor pollen tube discharge in gfa2/GFA2 ovules.