Publications

Selected Publications

Bold = Rhine Lab Members / * = Co-First Authors / † = Co-Corresponding Authors

Rhine, K., Li, R., Kopalle, H.M., Rothamel, K., Ge, X., Epstein, E., Mizrahi, O., Madrigal, A.A., Her, H-L., Gomberg, T.A., Hermann, A., Schwartz, J.L., Daniels, A.J., Manor, U., Ravits, J., Signer, R.A.J., Bennett, E.J., Yeo, G.W. Neuronal aging causes mislocalization of splicing proteins and unchecked cellular stress. Nature Neuroscience, 2025. https://www.nature.com/articles/s41593-025-01952-z
> Highlighted in https://www.nature.com/articles/s41593-025-01953-y


Rhine, K., Epstein, E., Carlson, N.M., Ge, X., Mizrahi, O., Kamat, A., Hermann, A., Brothers, W.R., Ravits, J., Bennett, E.J., Pekkurnaz, G., Yeo, G.W. RNA triggers chronic stress during neuronal aging. bioRxiv, 2025. https://www.biorxiv.org/content/10.1101/2025.08.04.668575v1


Rhine, K.*, Dasovich, M.*, Yoniles, J.*, Badiee, M., Skanchy, S., Ganser, L., Fare, C.M., Shorter, J., Leung, A.K.L.†, Myong, S.† Poly(ADP-ribose) drives condensation of FUS via a transient interaction. Molecular Cell, 2022. https://www.sciencedirect.com/science/article/pii/S1097276522000612.
> Highlighted in https://www.nature.com/articles/s41592-022-01599-9
> Highlighted in https://publichealth.jhu.edu/2022/a-collaboration-across-johns-hopkins-finds-a-passing-par-interaction-can-trigger-stable-fus-aggregations


Rhine, K., Vidaurre, V., Myong, S. RNA Droplets. Annual Reviews of Biophysics, 2020. https://www.annualreviews.org/content/journals/10.1146/annurev-biophys-052118-115508



Rhine, K., Makurath, M.A., Liu, J., Lopez, C., Catalan, K.F., Skanchy, S., Fare, C.M., Shorter, J., Ma, Y., Ha, T., Chemla, Y.R., Myong, S. ALS/FTLD-Linked Mutations in FUS Glycine Residues Cause Accelerated Gelation and Reduced Interactions with Wild-Type FUS. Molecular Cell, 2020. https://www.cell.com/molecular-cell/fulltext/S1097-2765(20)30721-8
> Highlighted in https://www.nature.com/articles/s42003-020-01560-6

All Publications
  1. Li, Y., Li, Z., Li, Y., Rhine, K., Ranamukhaarachchi, S., Qin, S., Jang, H., Villazon, J., Bai, Z., Fraley, S.I., Yeo, G.W., Fan, R., Shi, L. Imaging Cellular Metabolic Rewiring with SuMMIT-SRS. Available on bioRxiv: https://doi.org/10.1101/2025.10.10.681769. 
  2. Rhine, K., Epstein, E.*, Carlson, N.M.*, Ge, X., Mizrahi, O., Kamat, A., Hermann, A., Brothers, W.R., Ravits, J., Bennett, E.J., Pekkurnaz, G., Yeo, G.W. RNA triggers chronic stress during neuronal aging. Available on bioRxiv: https://doi.org/10.1101/2025.08.04.668575.
  3. Rhine, K., Li, R., Kopalle, H.M., Rothamel, K., Ge, X., Epstein, E., Mizrahi, O., Madrigal, A.A., Gomberg, T.A., Hermann, A., Schwartz, J.L., Daniels, A.J., Manor, U., Ravits, J., Signer, R.A.J., Bennett, E.J., Yeo, G.W. Aging-linked deterioration of RNA metabolism destabilizes the stress response of neurons. Nat Neurosci, 2025 Jun;28(6):1174-1184. doi: 10.1038/s41593-025-01952-z. Epub 2025 Jun 2.
    • Featured in RNA dysregulation impairs stress resilience in aged neurons. Nat Neurosci, 2025 Jun;28(6):1124-1125. doi: 10.1038/s41593-025-01953-y.
  4. Street, L.*, Rothamel, K.*, Brannan, K.*, Jin, W., Bokor, B., Dong, K., Rhine, K., Madrigal, A., Al-Azzam, N., Kim, J., Ma, Y., Abdou, A., Wolin, E., Doron-Mandel, E., Ahdout, J., Mujumdar, M. Jovanovic, M., Yeo, G.W. Large-scale map of the RNA binding protein interactomes across the RNA life-cycle. Mol Cell, epub ahead of print. doi:10.1016/j.molcel.2024.08.030.
  5. Liang, Q.*, Yu, T.*, Kofman, E., Jagannatha, P., Rhine, K., Yee, B., Corbett, K.D., Yeo, G.W. High-sensitivity in situ capture of endogenous RNA-protein interactions in fixed cells and primary tissues. Nature Communications, 2024 Aug 16; 7067.
  6. Rhine, K., Odeh, H.M., Shorter, J., Myong, S. Regulation of biomolecular condensates by poly(ADP-ribose). Chem Rev, 2023 Jul 26;123(14):9065-9093.
  7. Fare, C.M., Rhine, K., Lam, A., Myong, S., Shorter, J. Molecular determinants of Kapβ2 chaperone and disaggregation activity. J Biol Chem, 2022 Dec 15;299(2):102806.
  8. Rhine, K., Al-Azzam, N., Yu, T., Yeo, G.W. Aging RNA granule dynamics in neurodegeneration. Front Mol Biosci, 2022 Sep 16;9:991641.
  9. Rhine, K.*, Dasovich, M.*, Yoniles, J.*, Badiee, M., Skanchy, S., Ganser, L., Fare, C.M., Shorter, J., Leung, A.K.L, Myong, S. Poly(ADP-ribose) drives condensation of FUS via a transient interaction. Mol Cell, 2022 Mar 3;82(5):969-985.e11.
    • Featured in Seydel, C. Diving deeper into the proteome. Nat Methods 2022 Aug 25. doi: 10.1038/s41592-022-01599-9.
  10. Rhine, K., Myong, S. Single molecule probing of disordered RNA binding proteins. STAR Protocols, 2022 Jan 28;3(1):101131.
  11. Hui, M.H.*, Rhine, K.*, Tolan, D.R. Actin Filament- and Wiskott-Aldrich Syndrome Protein-Binding Sites on Fructose-1,6-bisphosphate Aldolase are Functionally Distinct from the Active Site. Cytoskeleton (Hoboken) 2021 Apr;78(4):129-141.
  12. Rhine, K., Skanchy, S., Myong, S. Single-molecule and ensemble methods to probe RNP condensate nucleation and properties. Methods, 2021 Feb 19:S1046-2023(21)00049-9.
  13. Rhine, K., Makurath, M.A., Liu, J., Lopez, C., Catalan, K.F., Skanchy, S., Fare, C.M., Shorter, J., Ma, Y., Ha, T., Chemla, Y.R., Myong, S. ALS/FTLD-Linked Mutations in FUS Glycine Residues Cause Accelerated Gelation and Reduced Interactions with Wild-Type FUS. Mol Cell, 2020 Oct 27;S1097-2765(20)30721-8.
    • Featured in Chattopadhyay, K. Probing the influence of mutations on FUS condensates, one molecule at a time Commun Biol, 2021 Jan 4;4(1):3.
  14. Rhine, K., Vidaurre, V., Myong, S. RNA Droplets. Ann Rev Biophys, 2020 May 6;49:247-265.
  15. Niaki, A.G.*, Sarkar, J.*, Cai, X., Rhine, K., Vidaurre, V., Guy, B., Hurst, M., Lee, J.C., Koh, H.R., Guo, L., Fare, C.M., Shorter, J., Myong, S. Loss of dynamic RNA interaction and aberrant phase separation induced by two distinct types of ALS/FTD-linked FUS mutations. Mol Cell, 2020 Jan 2;77(1):82-94.e4.

Funding Sources
Granting AgencyDateGrant IDProject
ALS Association2/2025 – 2/202626-SGP-764
(co-PI: Anne Bang/SBP)		High -throughput imaging screen to restore TDP-43 localization in aged neurons
ALS Association12/2022 – 12/202423-PDF-639Determining Changes in RNA and Protein Metabolism in ALS iMNs
NIH (NINDS)4/2020 – 5/20221F31NS113439-01A1Elucidating the molecular mechanism underlying ALS/FTD-linked FUS mutations with single-molecule resolution